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. 2021 Jun 17;8:697773. doi: 10.3389/fmolb.2021.697773

FIGURE 6.

FIGURE 6

SSH2 is the target gene of miR-378a-3p. (A) The potential mRNAs of miR-378a-3p from PITA, microT and miRanda under the condition of high stringency ≥ 3 on degradome data. (B) The expression of SSH2 or WRB in OS cells in NC mimics or miR-378a-3p mimics group was tested by RT-qPCR. Significance was analyzed by Student’s t-test. (C,D) The expression of SSH2 or WRB in OS cells transfected with NC inhibitor or miR-378a-3p inhibitor was examined by RT-qPCR. Significance was analyzed by Student’s t-test. (E) The expression of SSH2 in OS cell lines and the normal NHost cells was detected by RT-qPCR analysis. Significance was analyzed by Student’s t-test. (F) The expression of SSH2 in OS tissues and matched normal tissues was detected by RT-qPCR analysis. n = 42. Significance was analyzed by Student’s t-test. (G) The binding site between miR-378a-3p and SSH2 3′UTR. (H) The luciferase reporter assay was used to verify the binding ability between miR-378a-3p and SSH2 in OS cells. Significance was analyzed by Student’s t-test. (I) Western blot measured SSH2 protein level in OS cells transfected with NC mimics or miR-378a-3p mimics. Significance was analyzed by Student’s t-test. *p < 0.05.