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. 2021 Jun 17;12:675724. doi: 10.3389/fphys.2021.675724

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Copyright © 2021 Nomura, Nakano, Woo Sung, Han and Pandey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MLN4924 fails to increase acetylated tubulin in tubacin-pretreated HAECs. (A) HAECs were pretreated with tubacin for 4 h before addition of MLN4924 (1 μM). After an 18 h incubation, cells were immunoblotted (IB) with antibodies to acetylated α-tubulin, HDAC6, and total α-tubulin. (B,C) Densitometric analysis of HDAC6 and acetylated α-tubulin levels presented in (A). ^∗p < 0.05 vs. DMSO (control) group; n = 3.