TABLE 3.
Multiple reaction monitoring parameters of peptides (and internal standard) selected for targeted analysis of hepatobiliary transporters.
| Transports | Signature pedtides | Molecular weight | Parent ion (z = 2) | Product ion (z = 1) | Fragmentor | Collision energy |
|---|---|---|---|---|---|---|
| Mrp2 | LTIIPQDPILFSGSLR | 1770.08 | 885.7 | 1,329.9 | 200 | 25 |
| Oct1 | GVALPETIEEAENLGR | 1,697.84 | 849.7 | 1,357.8 | 180 | 29 |
| Ntcp | AAATEDATPAALEK | 1,358.42 | 680 | 915.5 | 140 | 18 |
| IS | AAATEDATPAALEK* | 1,366.42 | 684 | 923 | 140 | 23 |
| Oatp1a4 | TFQFPGDIESSK | 1,355.45 | 678.6 | 832.3 | 160 | 19 |
| Mate1 | LLLSGFQEELR | 1,304.48 | 652.9 | 965 | 140 | 24 |
| Bsep | STALQLIQR | 1,029.19 | 515.5 | 529.5 | 130 | 17 |
| Mdr1 | NTTGALTTR | 934.00 | 467.9 | 719.4 | 110 | 14 |
| Oatp1a1 | EENLGITK | 903.00 | 452.3 | 468.1 | 120 | 8 |
| Oatp1a2 | SVQPELK | 799.91 | 400.8 | 486.3 | 110 | 9 |
| UGT1A1 | TYPVPFQR | 1,336.48 | 504.5 | 547.1 | 140 | 19 |