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. 2020 Dec 15;11(6):1676–1685. doi: 10.1016/j.apsb.2020.12.007

Figure 4.

Figure 4

HPLC analysis for functional study of the P450 enzyme CepB4. (A) Three-gene combination transformant AOS10 with addition of helE, fusB1 and cepB4 into AOS0. (B) A. oryzae incubated with 5. (C) CO6 harboring cepB4 incubated with 5. (D) A. oryzae incubated with 39. (E) CO6 incubated with 39. (F) A. oryzae incubated with 37. (G) CO6 incubated with 37. (H) CepB4-mediated oxidation of C6 and C7. *The peak is annotated as the impurity peak on the basis of MS analysis (Supporting Information Fig. S76).