Table 2.
Real-time quantitative PCR primer sequences.
| Target gene | Primer sequence, forward (5′→3′) | Primer sequence, reverse (5′→3′) | Temperature, °C | Accession number |
|---|---|---|---|---|
| Hepcidin | AGCAGGAGCAGGATGAGC | GCCAGGGGATTTGTTTGT | 59.3 | JQ246442.1 |
| LEAP-2A | TGCCTACTGCCAGAACCA | AATCGGTTGGCTGTAGGA | 59.3 | FJ390414 |
| LEAP-2B | TGTGCCATTAGCGACTTCTGAG | ATGATTCGCCACAAAGGGG | 59.3 | KT625603.1 |
| β-defensin-1 | TTGCTTGTCCTTGCCGTCT | AATCCTTTGCCACAGCCTAA | 58.4 | KT445868.1 |
| Mucin2 | GAGTTCCCAACCCAACACAT | AAAGGTCTACACAATCTGCCC | 60.4 | KT625602 |
| IL-1β | AGAGTTTGGTGAAGAAGAGG | TTATTGTGGTTACGCTGGA | 57.1 | JQ692172 |
| IL-6 | CAGCAGAATGGGGGAGTTATC | CTCGCAGAGTCTTGACATCCTT | 62.3 | KC535507.1 |
| IL-8 | ATGAGTCTTAGAGGTCTGGGT | ACAGTGAGGGCTAGGAGGG | 60.3 | JN663841 |
| IL-12p35 | TGGAAAAGGAGGGGAAGATG | AGACGGACGCTGTGTGAGTGTA | 55.4 | KF944667.1 |
| IL-12p40 | ACAAAGATGAAAAACTGGAGGC | GTGTGTGGTTTAGGTAGGAGCC | 59.0 | KF944668.1 |
| IL-15 | CCTTCCAACAATCTCGCTTC | AACACATCTTCCAGTTCTCCTT | 61.4 | KT445872.1 |
| IL-17D | GTGTCCAGGAGAGCACCAAG | GCGAGAGGCTGAGGAAGTT T | 62.3 | KF245426.1 |
| TNF-α | CGCTGCTGTCTGCTTCA | CCTGGTCCTGGTTCACTC | 58.4 | HQ696609 |
| IFN-γ2 | TGTTTGATGACTTTGGGATG | TCAGGACCCGCAGGAAGAC | 60.4 | JX657682 |
| IL-4/13A | CTACTGCTCGCTTTCGCTGT | CCCAGTTTTCAGTTCTCTCAGG | 55.9 | KT445871.1 |
| IL-4/13B | TGTGAACCAGACCCTACATAACC | TTCAGGACCTTTGCTGCTTG | 55.9 | KT625600.1 |
| IL-10 | AATCCCTTTGATTTTGCC | GTGCCTTATCCTACAGTATGTG | 61.4 | HQ388294 |
| IL-11 | GGTTCAAGTCTCTTCCAGCGAT | TGCGTGTTATTTTGTTCAGCCA | 57.0 | KT445870.1 |
| TGF-β1 | TTGGGACTTGTGCTCTAT | AGTTCTGCTGGGATGTTT | 55.9 | EU099588 |
| TGF-β2 | TACATTGACAGCAAGGTGGTG | TCTTGTTGGGGATGATGTAGTT | 55.9 | KM279716 |
| JAK2 | AGAGGCCATCGAGAGCTACT | TCATACGCCCCAACTGCAA | 59.7 | JF825474.1 |
| TYK2 | TTCGCCGTGTGTTTGCAAA | ACGCCAAAATGAGGAGCCA | 59.7 | KT724353.1 |
| STAT1 | TGGCACTTTGGAACCAGCT | AGGAGTTCATGGAGCGCA | 59.5 | KU508677.1 |
| STAT3a | ACATTCCTGCTGCGCTTCA | ACGAGGATGTTGGTGGCAT | 59.8 | KC978890 |
| STAT3b1 | TCAACATGGCCCAGTGGAA | AGCGTTGCGTGAGATTCCT | 59.4 | KU559609 |
| STAT3b2 | TCAACATGGCCCAGTGGAA | AGCGTTGCGTGAGATTCCT | 59.4 | KU559610 |
| TOR | TCCCACTTTCCACCAACT | ACACCTCCACCTTCTCCA | 61.4 | JX854449 |
| β-actin | GGCTGTGCTGTCCCTGTA | GGGCATAACCCTCGTAGAT | 61.4 | M25013 |
LEAP-2 = liver expressed antimicrobial peptide 2; IL = interleukin; TNF-α = tumor necrosis factor α; IFN-γ2 = interferon γ2; TGF-β = transforming growth factor β; JAK2 = Janus kinase 2; TYK2 = tyrosine kinase 2; STAT = signal transducers and activators of transcription; TOR = target of rapamycin.