. 2021 Mar 11;7(2):481–487. doi: 10.1016/j.aninu.2020.09.005

Table 5.

Effects of Mn-MHAC on trace element deposition.¹

Item, μg/g	Dietary treatment						SEM	P-value	Polynomial contrasts, P-value
Item, μg/g	Control (0 mg Mn/kg)	Mn sulfate (100 mg Mn/kg)	Mn-MHAC (25 mg Mn/kg)	Mn-MHAC (50 mg Mn/kg)	Mn-MHAC (75 mg Mn/kg)	Mn-MHAC (100 mg Mn/kg)	SEM	P-value	Mn-MHAC linear	Mn-MHAC quadratic
Serum
Mn²	0.103^b	0.177^a	0.145^ab	0.156^a	0.113^b	0.172^a	0.0086	0.042	0.963	0.657
Cu³	0.212^c	0.203^c	0.359^bc	0.800^a	0.601^ab	0.614^ab	0.0516	<0.001	0.368	0.161
Fe	3.975	3.321	4.414	4.364	3.118	4.46	0.2387	0.497	0.811	0.722
Liver
Mn	2.676	2.843	2.677	2.859	3.068	2.939	0.0672	0.584	0.342	0.363
Cu⁴	4.075	3.975	4.693	4.758	4.615	4.863	0.1111	0.064	0.661	0.782
Fe	279.582	271.446	245.443	220.733	265.490	289.364	12.5936	0.639	0.160	0.377
Heart
Mn	0.465	0.418	0.446	0.423	0.412	0.436	0.0111	0.753	0.865	0.639
Cu	3.181	3.005	3.063	3.034	3.278	3.118	0.0479	0.610	0.539	0.742
Fe⁵	35.49	29.887	32.914	33.480	29.929	27.262	1.2923	0.120	0.020	0.052
Tibia
Mn⁶	1.789^b	2.127^b	1.992^b	2.179^b	3.046^a	2.990^a	0.1141	<0.001	0.002	0.009
Cu	2.077	2.140	2.262	2.043	2.154	2.587	0.0973	0.637	0.249	0.170
Fe⁷	70.757^a	54.914^b	60.633^ab	57.229^b	53.814^b	55.200^b	1.7235	0.029	0.286	0.475

Mn-MHAC = manganese methionine hydroxy analog chelated; Mn = manganese; Fe = iron; Cu = copper.

^{a, b, c} Values in the same row with different superscript are significantly different (P < 0.05) by one-way ANOVA.

Data are means of 6 replicates of 12 broilers per dietary treatment.

Serum Mn Regression equation: y = 0.009x + 0.101, R² = 0.103.

Serum Cu Regression equation: y = −0.028x² + 0.286x-0.072, R² = 0.305, P = 0.004 or y = 0.093x + 0.169, R² = 0.249.

⁴

Liver Cu Regression equation: y = 0.006x + 4.240, R² = 0.125.

⁵

Heart Fe Regression equation: y = 0.001x² - 0.146x + 38.855, R² = 0.292.

⁶

Tibia Mn Regression equation: y = 1.772x² + 0.012x + 1.733, R² = 0.419.

⁷

Tibia Fe Regression equation: y = 0.003x² - 0.414x + 70.441, R² = 0.282.