Table 4.
Primers used for gene quantitative RT-PCR.
| Gene | Accession No. | Ta, °C | Size, bp | Primer sequence (5′-3′) | Reference |
|---|---|---|---|---|---|
| ATP5A1W | XM_429118.5 | 60 | 232 | F-GGCAATGAAACAGGTGGCAG | Nafari (2019) |
| R-GGGCTCCAGCTTGTCTAAGTGA | |||||
| COX III | KC847880.1 | 60 | 72 | F-AGTCACCGTTACATGGGCTCA | Nafari (2019) |
| R-AGAGTTAGTGCGTGGATGGCTT | |||||
| HMBS | XM_417846.2 | 60 | 131 | F- GGCTGGGAGAATCGCATAGG | Yin et al. (2011) |
| R- TCCTGCAGGGCAGATACCAT | |||||
| HPRT | NM_204848.1 | 63 | 245 | F-ACTGGCTGCTTCTTGTG | Yang et al. (2013) |
| R-GGTTGGGTTGTGCTGTT | |||||
| ND2 | JQ970529.1 | 60 | 147 | F-AGGCTCCTCCCTAATCACTGC | Nafari (2019) |
| R-CCCATTCAGCCTCCGATTAG | |||||
| PGC-1A | XM 015285697.2 | 60 | 160 | AGATCACCGTACAGTCGCAGAC | This study |
| CACGACGCTCTTCAATTGCT | |||||
| SDHA | NM_001277398.1 | 60 | 74 | F-ATACGGGAAGGAAGGGGTTG | Nafari (2019) |
| R-TGCTGGGGTGGTAAATGGTG | |||||
| TBP | NM_205103 | 62 | 147 | F-TAGCCCGATGATGCCGTAT | Li et al. (2005) |
| D83127 | R- GTTCCCTGTGTCGCTTGC |
ATP5A1W = ATP synthase subunit alpha; COX III = cytochrome c oxidase subunit III; HMBS = hydroxymethylbilane synthase; HPRT = hypoxanthine phosphoribosyltransferase 1; ND2 = nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 2; PGC-1A = peroxisome proliferator-activated receptor gamma (PPAR-G) coactivator 1 alpha; SDHA = succinate dehydrogenase complex flavoprotein subunit A; TBP = TATA-box binding protein; Ta = annealing temperature; bp = base pair.