Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer sequences for real-time polymerase chain reaction.
| Gene | Primer sequence (5′ to 3′) | Size, bp |
|---|---|---|
| ZO-1 | F: TCAAGGTCTGCCGAGACAAC | 140 |
| R: ATCACAGTGTGGTAAGCGCA | ||
| Claudin-1 | F: ACAGGAGGGAAGCCATTTTCA | 82 |
| R: TTTAAGGACCGCCCTCTCCC | ||
| Occludin | F: CAGGTGCACCCTCCAGATTG | 111 |
| R: TGGACTTTCAAGAGGCCTGG | ||
| GAPDH | F: GAAGGTCGGAGTGAACGGAT | 149 |
| R: CATGGGTAGAATCATACTGGAACA |
ZO-1 = Zonula occludens-1; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.
