Table 2.
Primers used for quantitative real-time PCR.
| Gene name | Sequence (5′−3′) | Size, bp |
|---|---|---|
| SOD1 | F: GAGACCTGGGCAATGTGACT | 189 |
| R: CCAAACGACTTCCAGCATTT | ||
| GPX1 | F: AGCCCAACTTCATGCTCTTC | 159 |
| R: CATTGCGACACACTGGAGAC | ||
| NFE2L2 | F: GAAAGCCCAGTCTTCATTGC | 190 |
| R: TTGGAACCGTGCTAGTCTCA | ||
| GCLC | F: CAAACCATCCTACCCTTTGG | 172 |
| R: ATTGTGCAGAGAGCCTGGTT | ||
| HSL | F: GCAGCATCTTCTTCCGCACA | 195 |
| R: AGCCCTTGCGTAGAGTGACA | ||
| ACCα | F: ATCCCTCCTTGCCTCTCCTA | 208 |
| R: ACTTCCCGTTCAGATTTCCG | ||
| LPL | F: CTCGTGCTCAGATGCCCTAC | 148 |
| R: GGCAGGGTGAAAGGGATGTT | ||
| PPARγ | F: TGACCATGGTTGACACCG | 381 |
| R: AAGCATGAACTCCATAGTGG | ||
| FATP1 | F: GGAGTAGAGGGCAAAGCAGG | 208 |
| R: AGGTCTGGCGTGGGTCAAAG | ||
| PGC-1α | F: GCCCAGTCTGCGGCTATTT | 265 |
| R: GTTCAGCTCGGCTCGGATTT | ||
| Nrf2 | F: GCCCCTGGAAGCGTTAAAC | 59 |
| R: GGACTGTATCCCCAGAAGGTTGT | ||
| UCP2 | F: CTTCTGCGGTTCCTCTGTGT | 641 |
| R: CATAGGTCACCAGCTCAGCA | ||
| UCP3 | F: GAGATGGTGACCTATGATGT | 260 |
| R: CGCAAAAAGGAAGGTGTGAA | ||
| β-actin | F: TGCGGGACATCAAGGAGAAG | 216 |
| R: AGTTGAAGGTGGTCTCGTGG |
SOD1 = superoxide dismutase 1; F = forward; R = reverse; GPX1 = glutathione peroxidase 1; NFE2L2 = nuclear factor erythroid 2-like 2; GCLC = glutamate cysteine ligase catalytic subunit; HSL = hormone-sensitive lipase; ACCα = acetyl CoA carboxylase α; LPL = lipoprotein lipase; PPARγ = peroxisome proliferator-activated receptor γ; FATP1 = fatty acid transport protein 1; PGC-1α = peroxisome proliferator-activated receptor γ coactiva-tor-1α; Nrf2 = nuclear respiratory factor 2; UCP2 = uncoupling protein-2; UCP3 = uncoupling protein-3.