Table 8.
Relative mRNA expression levels of mitochondria respiratory chain related genes in skeletal muscle of Xiangcun black finishing pigs fed the diets with various levels of mulberry powder.
| Item | Mulberry inclusion level, % |
SEM |
P-value |
||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 3 | 6 | 9 | 12 | ANOVA | Linear | Quadratic | ||
| Longissimus dorsi muscle | |||||||||
| PGC-1α | 0.85 | 0.81 | 0.68 | 0.85 | 0.89 | 0.12 | 0.80 | 0.74 | 0.59 |
| Nrf2 | 1.53b | 1.43bc | 1.47bc | 1.96a | 1.05c | 0.15 | <0.01 | 0.44 | 0.15 |
| UCP2 | 0.93 | 0.70 | 0.91 | 0.79 | 1.12 | 0.11 | 0.11 | 0.20 | 0.08 |
| UCP3 | 0.99ab | 0.80b | 0.77b | 1.11ab | 1.23a | 0.13 | 0.08 | 0.07 | 0.03 |
| Biceps femoris muscle | |||||||||
| PGC-1α | 1.29b | 0.85b | 1.24b | 1.78a | 0.91b | 0.16 | <0.01 | 0.78 | 0.63 |
| Nrf2 | 1.25a | 0.53b | 0.83b | 0.71b | 0.62b | 0.11 | <0.01 | 0.01 | 0.01 |
| UCP2 | 0.99b | 0.92b | 1.66a | 1.18b | 1.21b | 0.15 | 0.01 | 0.20 | 0.11 |
| UCP3 | 0.65b | 0.54b | 0.58b | 0.71b | 1.39a | 0.11 | <0.01 | <0.01 | <0.01 |
PGC-1α = peroxisome proliferator-activated receptor γ coactiva-tor-1α; Nrf2 = nuclear respiratory factor 2; UCP2 = uncoupling protein-2; UCP3 = uncoupling protein-3.
a, b, c Within a row, values with different superscript letters differ (P < 0.05). n = 6.