Table 1.
The sequences of primers used for the real-time PCR analysis in the current study.
| Targeting gene or microorganism | Primer sequence (5′ to 3′) | References/Accession no. |
|---|---|---|
| Prevotella | F: CACCAAGGCGACGATCA | Bergström et al. (2012) |
| R: GGATAACGCCTGGACCT | ||
| Bacteroides | F: CGATGGATAGGGGTTCTGAGAGGA | Bergström et al. (2012) |
| R: GCTGGCACGGAGTTAGCCGA | ||
| β-Actin | F: TCTGGCACCACACCTTC | NM_001101 |
| R: TGATCTGGGTCATCTTC | ||
| GAPDH | F: TGAAGGTCGGAGTGAACGGAT | NM_001206359.1 |
| R: CACTTTGCCAGAGTTAAAAGCA | ||
| Clostridium cluster XIVa | F: AAATGACGGTACCTGACTAA | Mu et al. (2017) |
| R: CTTTGAGTTTCATTCTTGCGAA | ||
| Clostridium cluster IV | F: GCACAAGCAGTGGAGT | Mu et al. (2017) |
| R: CTTCCTCCGTTTTGTCAA | ||
| Faecalibacterium prausnitzii | F: CCCTTCAGTGCCGCAGT | Nielsen et al. (2014) |
| R: GTCGCAGGATGTCAAGAC | ||
| Butyryl-CoA:acetate CoA-transferase | F: GCIGAICATTTCACITGGAAYWSITGGCAYATG | Louis and Flint (2007) |
| R: CCTGCCTTTGCAATRTCIACRAANGC | ||
| Butyrate kinase | F: GTATAGATTACTIRYIATHAAYCCNGG | Louis et al. (2004) |
| R: CAAGCTCRTCIACIACIACNGGRTCNAC | ||
| SMCT1 | F: CGCAGATTCCTACTAACC | Haene et al. (2013a) |
| R: GATTGTCAGTTCCACCAT | ||
| MCT1 | F: CATCAACTACCGACTTCTG | Haene et al. (2013a) |
| R: TACTGGTCTCCTCCTCTT | ||
| Mucin 1 | F: GTGCCGCTGCCCACAACCTG | XM_001926883.4 |
| R: AGCCGGGTACCCCAGACCCA | ||
| Mucin 2 | F: GGTCATGCTGGAGCTGGACAGT | XM_003122394.1 |
| R: TGCCTCCTCGGGGTCGTCAC | ||
| OCLN | F: CTACTCGTCCAACGGGAAAG | NM_001163647.2 |
| R: ACGCCTCCAAGTTACCACTG | ||
| ZO-1 | F: CAGCCCCCGTACATGGAGA | XM_005659811 |
| R: GCGCAGACGGTGTTCATAGTT | ||
| GPR43 | F: GATCGTCTGTGCCCTCATGG | JX566881.1 |
| R: GAAAGCCACTCCCAGGTAGC | ||
| HDAC | F: TGACGAGTCCTATGAGGCCA | XM_013999116.2 |
| R: CAAACTCCACACACTTGGCG | ||
| IL-6 | F: GGGAAATGTCGAGGCTGTG | NM_214399 |
| R: AGGGGTGGTGGCTTTGTCT | ||
| IL-12 | F: CCTGACTGCCTCCCACTTTC | NM_214013 |
| R: AGGAGTGACTGGCTCAGAAC | ||
| IL-1β | F: ACGTGCAATGATGACTTTGTCTG | NM_214055.1 |
| R: AGAGCCTTCAGCATGTGTGG | ||
| TNF-α | F: CGTGAAGCTGAAAGACAACCAG | NM_214022.1 |
| R: GATGGTGTGAGTGAGGAAAACG |
GAPDH = glyceraldehyde-3-phosphate dehydrogenase; SMCT1 = sodium-coupled monocarboxylate transporter 1; MCT1 = monocarboxylate transporter 1; Mucin 1 = epithelial mucus 1; Mucin 2 = epithelial mucus 2; OCLN = epithelial tight junction protein occludin; ZO-1 = epithelial tight junction protein zonula occludens-1; GPR43 = G protein-coupled receptor 43; HDAC = histone deacetylases; IL-6 = interleukin-6; IL-12 = interleukin-12; IL-1β = interleukin-1β; TNF-α = tumor necrosis factor-α.