Figure 5.
Effect of compound 2 on the lipid mediator profile of activated blood. Lipid mediator profiles of human blood elicited by A23187 (A) or pathogenic E. coli (B) as determined by UPLC‒MS/MS. Heatmaps show mean percentage changes upon treatment with compound 2 or 5 relative to the vehicle control; zileuton (3 μmol/L). 20-OH-LTB4, 20-hydroxy-LTB4; HEPE, hydroxy-eicosapentaenoic acid; (di)HETE, (di)hydroxy-eicosatetraenoic acid; HDHA, hydroxy-docosahexaenoic acid; AA, arachidonic acid; EPA, eicosapentaenoic acid; DHA, docosahexaenoic acid. Line charts present data in pg/mL blood for selected lipid mediators as means + SEM, n = 3 (A), n = 4 (B). Data were log-transformed for statistical analysis. ∗∗∗P < 0.001 compared to vehicle control; repeated measures one-way ANOVA plus Tukey post hoc tests.