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. 2021 Feb 10;11(6):1578–1591. doi: 10.1016/j.apsb.2021.02.005

Figure 4.

Figure 4

LIX1L promotes hepatoma cell migration, invasion and metastasis. (A) and (B) Transwell assay analyzed effects of LIX1L on cell migration and invasion. scale bar = 100 μm. (C) and (D) EMT-related proteins were assessed by Western blot. (E) Silencing of LIX1L inhibited lung metastases in vivo. Bioluminescence imaging of mice 6 weeks after the tail vein injected with HCCLM3-shLIX1L#2-Luc or HCCLM3-Luc cells (n = 10 per group). Quantitative evaluation of bioluminescence from color scale. Four mice in control group and one mouse in HCCLM3-shLIX1L#2-Luc group died naturally under SPF conditions. (F) H&E staining of lung tissues obtained from nude mice. Arrow indicates lung metastatic nodules. Scale bar = 1 mm (left), 100 μm (right). (G) The number of lung metastatic foci (upper) and incidence of lung metastases (bottom). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.