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. 2021 Jul 1;11:99. doi: 10.1186/s13568-021-01255-z

Fig. 2.

Fig. 2

Optimization of the DPO system-based multiplex RT-PCR primer concentration. Lanes 1–8 represent the 8 primer concentrations 0.025 μM, 0.05 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.4 μM, 0.5 μM, and 0.6 μM; lane 9 represents negative control (N)