Fig. 4.

Detection specificity of SADS-CoV, TGEV, PEDV, PDCoV, and PRV-A by the DPO system-based multiplex RT-PCR. a A single pair of the DPO primer was used to amplify the mixture of the five plasmid templates. (Lanes 1–6: SADS-CoV, TGEV, PEDV, PDCoV, PRV-A, and negative control). b The mixture of the five DPO primers was used with each of the five plasmid templates individually, for amplification (Lanes 1–6: SADS-CoV, TGEV, PEDV, PDCoV, PRV-A, and negative control). c Detection of the other diarrhea viruses (Lanes 1–9: positive control (P), PRRSV, APPV, SVV, PCV3, PRCV, PTV, PPV, and negative template)