Fig. 5.

Detection sensitivity of SADS-CoV, TGEV, PEDV, PDCoV, and PRV-A by the DPO system-based multiplex RT-PCR. a Standard plasmids of TGEV, PEDV, PDCoV, SADS-CoV, and PRV-A were diluted tenfold to a final concentration between 10⁸ copies/μL and 10¹ copies/μL. The cDNAs were amplified with specific DPO primer sets. b The standard plasmids of each virus were incorporated after dilution and then added to a tube containing the DPO primer mixture for PCR amplification