Table 1. Primers sequence used for RT-PCR analysis.
| Gene (abbr.) | Gene (full name) | Sequence (5'-3') |
|---|---|---|
| OPG | Osteoprotegerin | F: tcactgggctgtttcttcag |
| R: tcctctttctcagggtgctt | ||
| RANKL | Receptor activator for nuclear factor-κB ligand | F: tcaggagttccagctatgat |
| R: ccatcagctgaagatagtcc | ||
| COL-I | Type I collagen | F: cagactggcaacctcaagaa |
| R: ggccaatgtctagtccgaat | ||
| RUNX-2 | Runt-related transcription factor 2 | F: tcaacatccgccaagagg |
| R: tacttgatgccacctgcgatg | ||
| OCN | Osteocalcin | F: gacaccatgaggaccatcttt |
| R: tagagaccactccagcaca | ||
| SOST | Sclerostin | F: cttcaggaatgatgccacagaggt |
| R: atctttggcgtcatagggatggtg | ||
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | F: gtgaggtgaccgcatcttct |
| R: cttgccgtgggtagagtcat |
OPG, osteoprotegerin; RANKL, receptor activator of nuclear factor kappa-B ligand; COL-I, collagen type I; RUNX-2, Runt-related transcription factor 2; OCN, osteocalcin; SOST, sclerostin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.