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. 2021 May;9(9):798. doi: 10.21037/atm-21-1838

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Characterization of exosomes and incorporation of exosomes into BMMs. (A) Electron microscopy images of exosomes. Scale bar, 100 nm. (B) The size distribution and concentration of exosomes secreted by BMSCs measured by nanoparticle tracking analysis. (C) Western blotting for the exosomal markers CD63 and TSG101, nuclear proteins LaminA/C and the endoplasmic reticulum protein Calnexin. (D) Confocal microscopy images showing the colocalization of exosomes from BMSCs with BMMs. Exosomes were labeled with DiO (green), and BMM cell nuclei were stained with Hoechst 33342 (blue). BMM, bone marrow macrophage; BMSCs, bone marrow mesenchymal stem cells; DiO, 3,3’-dioctadecyloxacarbocyanine perchlorate.