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. 2021 May;9(9):773. doi: 10.21037/atm-21-34

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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NEAT1 can stabilize the mRNA of TNFRSF1B. (A) The retrieved mRNA of TNFRSF1B from RNA-binding protein complexes obtained by RNA pull-down assay and analyzed by RT-qPCR. Biotin-NC probe is the negative control probe labeled with biotin. Biotin-NEAT1 probe is a biotin-labeled probe targeting NEAT1. (B) The predicted site that NEAT1 binds to the 3’UTR end of TNFRSF1B. (C,D) The mRNA expression of TNFRSF1B in NCM460 cells and HT-29 cells with time after the addition of actinomycin D, 18sRNA as internal reference because it is stable in the cell and is not affected by nuclease. ***P<0.001. NEAT1, nuclear enriched abundant transcript 1; TNFRSF1B, tumor necrosis factor superfamily member 1B; RT-qPCR, real-time quantitative polymerase chain reaction; TNF-α, tumor necrosis factor-alpha. NC, negative control.