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. 2021 May 5;40(13):e105770. doi: 10.15252/embj.2020105770

Figure 4. Wnt signalling induces TEAD1/2/4 mRNA expression in the intestine.

Figure 4

  1. RNAscope in situ hybridisation analysis of TEAD1/2/4 mRNA expression from control (wild type) and ApcMin mutant tumours from the small intestine (top) and colon (bottom). All three genes were strongly induced within the ApcMin mutant tumours, with TEAD2 being most strongly induced in colon ApcMin tumours (n = 3 animals with multiple tumours within the small and large intestines).
  2. qPCR analysis of control and Apc5 mutant murine intestinal organoids in vitro, control and ApcMin mutant tumour samples in vivo, and control and Apcfl / fl knockout hyperplastic crypts in vivo. In all cases, TEAD1/2/4 mRNA expression was strongly elevated upon loss of Apc function, while TEAD3 mRNA expression was unaffected. (RNA was isolated from six individual tumours). *P < 0.05; ****P < 0.0001.
  3. Antibody staining for TEAD4 reveals a gradient of expression along the crypt‐villus axis in both the small and large intestine, with uniformly high expression in colorectal adenomas, similar to SOX9.