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. 2021 Jun 18;14(6):dmm048901. doi: 10.1242/dmm.048901

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Schematic of the mutagenesis, breeding and screening strategy. Mutagenized G0 mice (ENU) are outcrossed to C57BL/6J (BL6) females. G1-generation pups are outcrossed, and subsequent G2-generation mice are backcrossed to their G1 sire to produce pedigrees of G3 mice for screening. Dorsal and lateral radiographs are obtained for all G3 mice, and mice are visually scored as affected or unaffected. Arrows indicate a spine deformity. Automated meiotic mapping tests whether ENU alleles are associated with the spine deformity phenotype after correction for the number of ENU alleles tested in the pedigree. Scale bars: 1 cm.