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. 2021 Jun 30;9(6):e002426. doi: 10.1136/jitc-2021-002426

Figure 3.

Figure 3

Tc17-1 and Tc1 cells are equally efficacious when combined with TMZ, poly-ICLC, and the peptide vaccine. C57BL/6 mice bearing intracranial GL261-Quad glioma received TMZ treatment on day 9, then a single IV infusion of Tc1 or Tc17-1 cells on day 10, followed by poly-ICLC and the peptide vaccine on day 11 after tumor inoculation. (A) Kaplan-Meier survival curve (n=10 per group). Data are pooled from two independent experiments. ***p<0.0001; log-rank test. (B–D) GL261-Quad-bearing mice in each treatment group were analyzed on day 13 (n=3 per group). Frequency of VLA-4- (B) and IFN-γ-expressing cells (C) on Pmel-1-derived infused Tc1 and Tc17-1 cells in cervical lymph nodes (CLNs) and spleen, respectively. Frequency of CD90.1+ cells on CD8+ T-cells in the spleen (D). Data are representative of 2–3 independent experiments and presented as mean±SD. *P<0.05, **p<0.001, ****p<0.00001; one-way ANOVA with interaction followed by Tukey’s multiple comparison test. ns, not significant. (E) Representative flow cytometry data showing the frequency of CD8+CD90.1+ cells in BIL at around day 25 after adoptive transfer (n=2 per group). ANOVA, analysis of variance; BIL, brain-infiltrating lymphocytes; TMZ, temozolomide.