图 1.

原代分离的CAFs和PTFs形态及表型鉴定。A：显微镜下观察CAFs及PTFs的细胞形态（×100）；B：免疫荧光检测α-SMA及FAP在CAFs和PTFs中的表达；C：免疫组化检测α-SMA在CAFs和PTFs中的表达量（×100）。

Morphology and phenotypic identification of primary isolated CAFs and PTFs. A: Morphological features of primary cultured PTFs and CAFs (×100). B: The expression levels of α-SMA and FAP in CAFs and PTFs were analyzed by immunofluorescence technique. The slide was stained with 4', 6-diamidino-2-phenylindole (DAPI, blue), FAP antibody (green) and α-SMA antibody (red); C: The expression levels of FAP in CAFs and PTFs were analyzed by immunohistochemistry (×100). CAFs: cancer-associated fibroblasts; PTFs: peri-tumer fibroblasts; α-SMA: alpha-smooth muscle actin; FAP: fibroblast activation protein.