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. 2020 Oct 26;109(6):864–876. doi: 10.1002/jbm.b.34751

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© 2020 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals LLC.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The effect of different ratios of cross‐linked HA to naïve HA on permeability. (a) Measurement of TEER on T84 cells treated with different ratios of cross‐linked HA to naïve HA at a HA concentration of 1 mg/ml HA on T84 cells pretreated with protamine sulfate and TNFα mixture (b) Measurement of TEER on T84 cells treated with different ratios of cross‐linked HA to naïve HA at a HA concentration of 3 mg/ml HA on T84 cells pretreated with protamine sulfate and TNFα mixture. (c) Measurement of Papp on the effect of different ratios of cross‐linked HA to naïve HA at both 1 and 3 mg/ml over 6 hrs on T84 cells pretreated with protamine sulfate and TNFα mixture *p < 0.05 compared to basal cells at same time point. (Basal‐healthy/uninflamed cells; Control‐protamine sulfate and TNFα mixture pretreated), N = 3