Figure 5.

Comparison of transcriptomic aging rate in conventional vs axenic conditions

Age-classifier genes selected separately in conventional or axenic conditions were compared and shared classifiers were identified. A linear model for transcriptomic age (calculated age) was developed from gene expression data of conventionally raised flies using the shared classifiers, and this model was applied to calculate the effective transcriptomic age of axenic flies. Conventional samples: replicates are gray circles; mean value is a purple circle; SD as indicated. Axenic samples: replicates are red circles; mean value is a blue square; SD as indicated. Black line is the regression line for calculated vs chronological age for conventionally raised samples; dashed red line is the regression line for axenic samples. The significance of the difference in slope was calculated with GraphPad Prism using linear regression analysis. The full list of shared aging classifier genes is in Table S12.