Figure 1.

Impact of speed and time on homogenization

Representative images of samples each consisting of 5 whole Drosophila homogenized at different speeds and for different time intervals in 150 μL PBS. After homogenization, the samples were briefly centrifuged for 15 s to bring down debris. In the upper panel, the samples are homogenized at maximum speed (10) but for a variable homogenization time (0 s, 10 s, 1 min, 3 min, 5 min). Note the presence of floating, unhomogenized tissues when the homogenization is run for only 10 s. Samples appear to be well homogenized after 3 and 5 min. In the lower panel, the samples are homogenized for a maximum time (5 min) but at variable speeds (0, 2, 4, 6, 8, and 10). Note the presence of unhomogenized tissues (such as red fly eyes) at speed 2 and 4. Samples appear to be well homogenized at speed 6, 8, and 10. Homogenize at the maximum speed (10) and time (5 min) for best results.