Figure 2.

Age-related increase in detergent-soluble and -insoluble polyubiquitinated proteins in Drosophila

Aging leads to an increase in poly-ubiquitinated proteins found in detergent-insoluble (and to a lower extent in detergent-soluble) fractions of Drosophila tissues, such as the fly thorax (which consists primarily of skeletal muscle) and the head (which primarily consists of retina and brain). Age-related increase in poly-ubiquitinated proteins is accompanied by parallel increases in the levels of Ref(2)P, the Drosophila homolog of p62/SQSTM1. Moreover, the ratio of LC3/Atg8-II (lower band) versus LC3/Atg8-I declines with aging, which is presumably indicative of an age-related decline in the autophagic flux; β-actin and/or α-tubulin are shown as normalization controls. The quantitation of ubiquitin (full lane) to the α-tubulin or β-actin band is shown in colored boxes. This data is taken from reference (Rai et al., 2021).