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. 2021 Jun 19;45:102040. doi: 10.1016/j.redox.2021.102040

Fig. 8.

Fig. 8

mRNA expression levels of autophagy markers in skeletal muscles of mdx mice treated with NaHS. (A) Heatmap representation of selected genes obtained from PCR array analysis performed in QFA muscles isolated from 7-week-old control (n = 6) and mdx mice treated with vehicle (n = 6) or NaHS (n = 6). Red, physiological gene expression; green, gene downregulation. (B–E) Bar graphs showing the mRNA expression levels of Atg3, Atg7, Atg12 and Ulk1 in quadriceps femoris of 7 weeks old control and mdx mice receiving or not NaHS. Data are expressed as 2∧-ΔΔct relative to actin, as described in materials and methods. (F–I) Western blot analysis showing the changes in the expression and/or phosphorylation of the two markers of autophagy/apoptosis AKT and LC3 in QFA of control and mdx mice treated or not with NaHS. Fold data represent the mean ± SEM of three separate experiments. The blots shown are representative of three independent experiments with similar outcomes. The GAPDH bands confirm that similar amounts of proteins were loaded on the gel for each sample. Single asterisk (*), double asterisk (**) and triple asterisk (***) denote a p-value of ≤0.05, 0.01, 0.001 vs wt mice, respectively. Single circle (○), double circle (○○) and triple circle (○○○) denote a p-value of ≤0.05, 0.01, 0.001 vs mdx group, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)