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. 2021 Jun 30;19:283. doi: 10.1186/s12967-021-02946-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The level of phosphorylated STAT3 of HPMCs ex vivo in drained PD effluent. A HPMCs were cultured from drained PD effluent of two patients (patient 1: PD duration 1 month; patient 3: PD duration 3 years). Then cellular localization of p-STAT3 (green) in HPMCs was detected using immunofluorescence analysis. Original magnification, ×200. B Western blot was performed with PD effluent cell lysates centrifuged from patients with different PD duration. The levels of Fibronectin and p-STAT3 were present in these long-term patients (No. 2, 3, 4; PD duration 1 year, 3 years and 6 years, respectively) but not in a new patient (No. 1; PD duration 1 month)