Fig. 7.

Effect of STAT3 phosphorylation blockade on level of HIF-1α and fibrosis related proteins in peritoneum. Peritoneal membrane of mice was collected at 28 days after 4.25% HG-PDF injection with or without administration of S3I-201 (10 mg/kg/day). A Peritoneal membrane lysates were subjected to examine the mRNA of Collagen I, α-SMA, HIF-1α and TGF-β1 using real-time PCR. B, C Peritoneal membrane lysates were subjected to immunoblot analysis with specific antibodies against HIF-1α and GAPDH; the representative results with three samples were shown. Data were expressed as the mean ± SE of three experiments. P values were determined by t tests. *P < 0.05; **P < 0.01; ***P < 0.001; ns: not significant