Table 2.
Primers for mutagenesis and RT-qPCR in this study
| Primer | Sequencea |
|---|---|
| Mutagenesis | |
| pCD163-G499R, forward | GTACAACATGGAGACACGTGGcgcACCGTCTGTGATTCTGAC |
| pCD163-G499R, reverse | GTCAGAATCACAGACGGTgcgCCACGTGTCTCCATGTTGTAC |
| pCD163-E509H, forward | GTGATTCTGACTTCTCTCTGcacGCGGCCAGCGTGCTGTGC |
| pCD163-E509H, reverse | GCACAGCACGCTGGCCGCgtgCAGAGAGAAGTCAGAATCAC |
| pCD163-S512N, forward | GACTTCTCTCTGGAGGCGGCCaacGTGCTGTGCAGGGAACTAC |
| pCD163-S512N, reverse | GTAGTTCCCTGCACAGCACgttGGCCGCCTCCAGAGAGAAGTC |
| pCD163-T522D, forward | GAACTACAGTGCGGCgacGTGGTTTCCCTC |
| pCD163-T522D, reverse | CAGGAGGGAAACCACgtcGCCGCACTGTAGTTC |
| pCD163-E534K, forward | CTGGGGGGAGCTCACTTTGGAaaaGGAAGTGGACAGATCTG |
| pCD163-E534K, reverse | CAGATCTGTCCACTTCCtttTCCAAAGTGAGCTCCCCCCAG |
| pCD163-E543K, forward | GTGGACAGATCTGGGCTGAAaaaTTCCAGTGTGAGGGGCACGAG |
| pCD163-E543K, reverse | CTCGTGCCCCTCACACTGGAAtttTTCAGCCCAGATCTGTCCAC |
| pCD163-H549S, forward | GAAGAATTCCAGTGTGAGGGGagcGAGTCCCACCTTTCACTCTG |
| pCD163-H549S, reverse | CAGAGTGAAAGGTGGGACTCgctCCCCTCACACTGGAATTCTTC |
| pCD163-P560Q, forward | CTTTCACTCTGCCCAGTAGCAcaaCGCCCTGACGGGACATGTAGC |
| pCD163-P560Q, reverse | GCTACATGTCCCGTCAGGGCGttgTGCTACTGGGCAGAGTGAAAG |
| pCD163-R561H, forward | CACTCTGCCCAGTAGCACCCcacCCTGACGGGACATGTAGCCAC |
| pCD163-R561H, reverse | GTGGCTACATGTCCCGTCAGGgtgGGGTGCTACTGGGCAGAGTG |
| pCD163-G564D, forward | CAGTAGCACCCCGCCCTGACgacACATGTAGCCACAGCAGGGAC |
| pCD163-G564D, reverse | GTCCCTGCTGTGGCTACATGTgtcGTCAGGGCGGGGTGCTACTG |
| pCD163-E534K/R561H, forward | CGGCGTAGTCTGCTCAAGATACACACAAATC |
| pCD163-E534K/R561H, reverse | CTCCAACCAGCCTGGGTTTCCTGTGGGCTG |
| RT-qPCR | |
| PRRSV-ORF7, forward | AAACCAGTCCAGAGGCAAGG |
| PRRSV-ORF7, reverse | GCAAACTAAACTCCACAGTGTAA |
| GAPDH, forward | CCTTCCGTGTCCCTACTGCCAAC |
| GAPDH, reverse | GACGCCTGCTTCACCACCTTCT |
aThe lowercase letters indicate mutated sites.