FIGURE 7.

Summary of DNA structures predicted by agarose gel location and enzymatic analyses. (A) After PFGE, the loading well contains complex/circular forms of the mitochondrial genome and a small proportion of nuclear chromosomes and their replication intermediates. (B) The prominent band contains linear megabase‐sized chromosomes 1–14. Under these PFGE conditions, individual chromosomes are not resolved. (B*) In highly resistant parasites, the size of chromosome 6 varies due to the presence of tandemdhodhamplicons (blue boxes). (C) Kilobase‐sized nuclear DNA fragments migrate below the chromosomes; their size varies due to stochastic breakage during electrophoresis. (D) In highly resistant parasites, the loading well contains complex/circular forms of ecDNA and accompanying replication intermediates, which may contain stretches of fragile linear DNA. (E) The low migrating “smear” contains linear ecDNA elements that break from complex/circular forms during electrophoresis. WT1, Dd2; H1, high‐level resistant clone; M, marker