Table 1.

Diagnostic Process, Number of Subjects, and Results

Groups	Diagnostic Process
	1, Serological Screening	2, Serological Validation	3, Parasitological Confirmation	4, Staging		No. of Subjects
	CATTwb / RDT	CATTpa	mAECT BC / LN aspirate observation	Parasites in CSF	No. WBCs in CSF	Screened	Enrolled	Followed Up
Seronegative	−	ND	ND	ND		5377	40	0
	+	<1/4
Seropositive	+	≥1/4	−	ND		12	8	5
Confirmed	+	≥1/4	+
Stage 1				No	0–5	8	4	4
Stage 2				Yes	>5	18	14	13
ND				ND		2	0	0
All						28	18	17
Total						5417	66	22

Abbreviations: CATTp, card agglutination test for trypanosomiasis on plasma; CATTwb, card agglutination test for trypanosomiasis on whole blood; CSF, cerebrospinal fluid; mAECT BC/LN aspirate, mini anion-exchange column technique on buffy coat/lymph node aspirate; ND, not determined; WBC, white blood cell.

^aHighest plasma dilution with a positive result.