FIGURE 9.

A673T mutant protein is more potent at inducing vesicle trafficking deficits than wt. Wt and mutant Aβ(1‐42) protein oligomer potencies were compared via vesicle trafficking assay. Mature primary neurons, 21 days in vitro, were treated for 24 hr with a particular protein oligomer at varying concentrations as indicated in the plot. The EC₅₀ for A673T mutant and wt protein oligomers were 2.22 and 4.73 μM, respectively (variable slope sigmoidal dose–response curve fit was used to fit the data and an extra sum of squares F‐test was performed to show difference in potency, p < .001). These results indicate that the A673T mutant protein oligomers are more potent than wt protein oligomers at inhibiting vesicle trafficking. All data represent mean ± SEM from N = 16 independent neuronal culture experiments