Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 4;21(5):1740–1753. doi: 10.1111/ajt.16334

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors. American Journal of Transplantation published by Wiley Periodicals LLC on behalf of The American Society of Transplantation and the American Society of Transplant Surgeons

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Kidney biopsy retrieval and proteomics workflows used in this study. Discarded kidneys were subjected to normothermic perfusion (NMP) either with urine recirculation (URC) or urine replacement (UR), and kidney cortex tissue biopsies collected at time points between 0 and 24 hours. Tissue material was homogenized, lysed, digested with trypsin using a filter‐assisted sample preparation (FASP) protocol followed by liquid chromatography tandem mass spectrometry (LC‐MS/MS) label‐free quantitative analysis (LFQ) and data processing using MaxQuant and Perseus software