Fig. 5.

Effects of FMNL2 on the nuclear levels of p27 and the degradation of p27. a Cellular fractionation experiments were performed to examine the levels of FMNL2 and p27 protein in the nucleus and cytoplasm. GAPDH and Lamin A/C were used as loading controls. b Representative fluorescent images of p27 were displayed. c After transfection for 48 h, cultured cells were incubated with cyclohexmide (CHX, 100 μg/mL) or MG132 (5 μM) for the indicated times. The levels of p27 protein were detected by western blotting. d A plot of normalized p27 expression is shown. e The ubiquitination of p27 was detected by western blotting. Scale bar, 50 μm