Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 30;22:195. doi: 10.1186/s13059-021-02406-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — ecDNA detection in K562 cells. a Diagram showing the detection of ecDNAs by SMOOTH-seq. The green part and orange part show the flanking regions of the circulation sites. The Tn5 transposases bind on an ecDNA molecule, generates linear fragments spanning the circulation site. When only one copy of Tn5 transposases inserts into an ecDNA, it will produce linearized read sequence of the full ecDNA length. b The location distribution of candidate ecDNAs (yellow) and PCR verified ecDNAs (purple) on the chromosomes (R package chromoMap v0.2 was used for figure plotting). The stars indicate regional overlap of both unvalidated and validated ecDNAs. c The length distribution of ecDNAs (125 in total) detected in K562 cells. d Validation of six ecDNAs in K562 cells by full-length PCR using divergent primers as indicated in the bottom. ecDNA#1 represents chr8: 70,427,292-70,432,578, ecDNA#2 represents chr6:117,278,014-117,283,920, ecDNA#3 represents chr19:4,218,299-4,224,954, ecDNA#4 represents chr19:58,198,975-58,213,955, ecDNA#5 represents chr2:140,078,412-140,091,946, ecDNA#6 represents chr6:35,786,783-35,799,008. e Sanger sequencing result of the circularization site of ecDNA#6 in panel d