Table 4.
scRNA-seq gene signatures used for FAP identification and clustering in muscle homeostasis
| Genes/markers | FAP subpopulations | Species | Reference |
|---|---|---|---|
| Sca1, Cd34, Pdgfra | SubFAPs: Tie2low (Tek), Vcam1low | Mouse | [62] |
| SubFAPs: Tie2high (Tek), Vcam1high | |||
| Ly6a (Sca1), Ly6e, Pdgfra, Dcn | Not determined | Mouse | [58] |
| Pdgfra, Ly6a (Sca1), Hic1 | FAP1: Cxcl14, Col4, Col6, Col15, Lum, Sparcl1, Podn, Smoc2, Mgp, and Bgn | Mouse | [48] |
| FAP2: Dpp4, Sfrp4, Igfbp5, Sema3c, Tgfrb2, and Wnt2 | |||
| Pdgfra, Ly6a (Sca1), Dcn, Cd34 | Not determined | Mouse | [216] |
| Pdgfra, Col3a1, Dcn, and Gsn | Not determined | Mouse | [107] |
| Pdgfra, Ly6a (Sca1), Cd34 | FAP1: Cxcl14, Enpp2 (Autotaxin), Crispld2, Hsd11b1, Smoc2, Ccl11, Gsn, and Dcn | Mouse | [108] |
| FAP2: Dpp4, Pi16, Wnt2, Igfbp5, Igfbp6, Fbn1, and Ugdh | |||
| PDGFRA, CD34, COLLAGEN 1, COLLAGEN 3, and COLLAGEN 6 | LUMICAN (LUM) FAP: LUM, DCN, CXCL14, COLLAGEN 4, and COLLAGEN 15, SMOC2, and GSN | Mouse and human | [109] |
| FIBRILLIN 1 (FBN1) FAP: FNB1, MFAP5, LOXL1, PRG4, ELN, IGFBP5, and FSTL1 | |||
| PDGFRA | FAP1 (fibroblasts 1): COL1A1, SFRP4, SERPINE1, and CCL2 | Human | [106] |
| FAP2 (Fibroblast 2): FBN1, MFAP5, and CD55 | |||
| FAP3 (Fibroblast 3): SMOC2, ADH1B, and ABC18 | |||
| PDGFRA, CD34, COL1A1, COL6A3, TCF7L2 | FAP1: PCOLCE2, MFAP5, IGFBP6, ENNP1, CD55, and AXL | Human | [16] a |
| FAP2: LUM, MYOC, CCL2, ADH1B, SFRP2, CXCL14, and MGP | |||
| FAP3: TNXB, C3, COL15A1, SMOC2, ABCA8, COL6A1, and HMCN2 | |||
| FAP4: IGF1, CRLF1, SCN7A, ITIH5, PTGDS and NOV | |||
| FAP5: SEMA3C, PRG4, DEFB1, CCDC80, LINC01133, and IGFBP5 |
aBy re-clustering the FAP population, the authors described the existence of 7 different FAP subpopulations in human muscles [16]