Figure 2.

Virus inactivation by S/D mixes containing TX‐100 versus compound II (TX‐100 reduced). A, Plasma‐derived model matrix was spiked with PRV before the addition of S/D mix consisting of PS80, TNBP, and TX‐100 or compound II. Duplicate runs were performed at 17°C at a final S/D mix concentration of 5% as specified for manufacturing. B, Recombinant model matrix was spiked with Xenotropic murine leukemia virus (X‐MuLV) before the addition of S/D mix consisting of PS80, TNBP, and TX‐100 or compound II. Duplicate runs were performed at 1°C at a final S/D mix concentration of 10% as specified for manufacturing. A, B, Samples were drawn after 1, 10, 30, and 60 min; viral loads of these samples were compared to a sample drawn before S/D mix addition to calculate the respective RF. Asterisks indicate viral inactivation below the detection limit. SD only shown if larger than the height of symbols). PRV, pseudorabies virus; RF, reduction factor; S/D, solvent/detergent; SD, standard deviation; TNBP, tri‐n‐butyl‐phosphate; TX‐100, Triton X‐100