Figure 4.

Virus inactivation by S/D mixes containing novel compounds IV and V. Duplicate runs were performed at 17°C using a plasma‐derived model matrix and PRV. PS80 and TNBP were combined with (A) compound IV or (B) compound V and added to the virus‐spiked matrix to reach a final concentration of 5% as specified for manufacturing. A, B, Samples were drawn 1, 10, 30, and 60 min after S/D mix addition; viral loads of these samples were compared to a sample drawn before S/D mix addition to calculate the respective reduction factor (RF). Asterisks indicate viral inactivation below detection limit. SD only shown if larger than the height of symbols). PRV, pseudorabies virus; RF, reduction factor; S/D, solvent/detergent; SD, standard deviation; TNBP, tri‐n‐butyl‐phosphate; TX‐100, Triton X‐100