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. 2021 Jun 11;12(16):4780–4790. doi: 10.7150/jca.58250

Figure 2.

Figure 2

Downregulation of ALDH3A1 inhibits proliferation in lung cancer cells. (A) Expression of ALDH3A1 in normal lung cell line BEAS-2B and lung cancer cell lines A549 and Calu3. Knockdown efficiency of ALDH3A1 in A549 cell line transduced with ALDH3A1-sh1 and ALDH3A1-sh2 by real-time PCR (B) and western blot (C). (D) Mean fluorescence intensity (MFI) of Ki67 in A549 cell line transduced with ALDH3A1-sh1 and ALDH3A1-sh2 was detected by flow cytometry. (E) Tumor cell growth from A549 cell line transduced with ALDH3A1-sh1 and ALDH3A1-sh2 by CCK-8, and absorbance per well was measured at 450 nm using an automatic microplate reader. Data were expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.