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. 2021 Jun 11;12(16):4819–4829. doi: 10.7150/jca.58670

Figure 1.

Figure 1

ECM detachment upregulates V-ATPase expression in tumor cells. (A) Hierarchical clustering of differentially expressed genes between HELA cell cultured in suspension (3 and 6 hours) and adherent HELA cell obtained after cell passage; (B-C) Relative mRNA levels of some upregulated V-ATPase in HELA (B) and MCF-7 (C) cultured in suspension or adherent conditions for 6 hours. Data represent means ± SEM, ** p< 0.01, ***p <0.001, ****p < 0.0001, ns, no significant difference; the experiments were repeated at least three times with similar results. (D) Lysates of HELA, MCF-7, and B16F10 cells cultured in adherent (ATT) and suspension (SUS) conditions for 6 hours were evaluated for ATP6V1B2 and ATP6V0D1 by western blot.