Figure 3.

Scavenging ROS reduced V-ATPase-induced anoikis and misfolded protein accumulation. (A) Percentage apoptotic HELA, MCF-7, and B16F10 cells treated with or without 10 nM bafilomycin A and 2 mM NAC for 24 hours as determined by flow cytometry; (B) Mean fluorescence intensity of HELA, MCF-7, and B16F10 treated with or without bafilomycin A and NAC for 24 hours, stained with CM-H₂DCFDA for 30 minutes and evaluated by flow cytometry; (C) K48 polyUb-modified proteins in ECM-detached HELA, MCF-7 and B16F10 cells treated with or without bafilomycin A and NAC for 24 hours; SS and NS fractions are shown. NS - NP40 soluble fraction, SS - SDS soluble fraction (D) Percentage apoptotic HELA and MCF-7 cells treated with or without 5 nM concanamycin A and 2 mM NAC for 24 hours as determined by flow cytometry. (E) Mean fluorescence intensity of HELA and MCF-7 treated with or without concanamycin A and NAC for 24 hours, stained with CM-H₂DCFDA for 30 minutes, and evaluated by flow cytometry. Data are expressed as means ± SEM. Representative data from three independent experiments (D-E). One way ANOVA: p*<0.05, **p < 0.01, ***p < 0.001, ****p< 0.0001, ns, no significant difference.