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. 2021 Jun 16;12(16):5053–5065. doi: 10.7150/jca.57752

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SOX4 knockdown inhibited EMT and CSC phenotype in BT-549 cells. (A) Morphological changes of cells with or without SOX4 knockdown. EMT morphological changes were induced by TGF-β. Scale bar, 100 µm. (B) Western blot results of EMT related markers. (C) Immunofluorescence staining results of EMT related markers. (D) Phase contrast images of mammospheres formation. Scale bar, 100 µm. (E) Flow cytometry assays of expression of CD44 and CD24 in cells transfected with shSOX4 or empty vector. (F) ALDEFLUOR assay. Inhibition of SOX4 significantly decreased ALDH⁺ cell population. The error bars indicate the standard errors of the mean from three independent experiments. *** P < 0.001.