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. 2021 Jun 16;12(16):5053–5065. doi: 10.7150/jca.57752

Figure 4.

Figure 4

SOX4 was required for miR-30a mediated inhibition of EMT and CSC phenotypes. (A) Western blot results of EMT related markers in MCF-7 transfected with miR-30a and SOX4 lacking of 3'UTR. (B) Phase contrast images of mammospheres formation. Scale bar, 100 µm. (C) Flow cytometry assay of breast cancer stem cell markers CD44 and CD24. (D) ALDEFLUOR assay. The error bars indicate the standard errors of the mean from three independent experiments. ** P < 0.01.