Table 2.
Primers used for quantitative real-time PCR.
| Gene | Accession No. | Primer, 5′-3′ | Size (bp) | TA (°C) |
|---|---|---|---|---|
| β-actin | XM_021086047.1 | F: CTGCGGCATCCACGAAACT | 147 | 61 |
| R: AGGGCCGTGATCTCCTTCTG | ||||
| IFN-γ | NM_213948.1 | F: GCCATTCAAAGGAGCATGGA | 144 | 58 |
| R: TTCACTGATGGCTTTGCGCT | ||||
| TNF-α | NM_214022.1 | F: CCCCTGTGAGGGCAGGA | 185 | 60 |
| R: CAGGCCACACATCCCTGAAT | ||||
| IL-1β | NM_214055.1 | F: CCTGAGATTGATGCCGTCCA | 267 | 60 |
| R: TCTTCAAGCCGTGTAGCCAT | ||||
| IL-6 | NM_214399.1 | F: CCTGAGATTGATGCCGTCCA | 267 | 59 |
| R: TCTTCAAGCCGTGTAGCCAT | ||||
| Claudin-1 | NM_001244539.1 | F: AAGGACAAAACCGTGTGGGA | 247 | 60 |
| R: CTCTCCCCACATTCGAGATGATT | ||||
| Occludin | NM_001163647.2 | F:ACGAGCTGGAGGAAGACTGGATC | 238 | 60 |
| R:CCCTTAACTTGCTTCAGTCTATTG | ||||
| ZO-1 | XM_021098896.1 | F: CCTGCTTCTCCAAAAACTCTT | 252 | 60 |
| R: TTCTATGGAGCTCAACACCC | ||||
| IKKβ | NM_001099935.1 | F: GTGACATCGCCTCTGCACTT | 81 | 59 |
| R: GCAGGACGATGTTTTCTGGC | ||||
| IκBα | XM_001924394.6 | F: CACCCGAGTTAGAAGGGCTC | 155 | 59 |
| R: GGTATCTGCTGAGGTGTGCTG | ||||
| NF-κB | NM_001048232.1 | F: AGCCATTGACGTGATCCAGG | 248 | 60 |
| R: CGAAATCGTGGGGCACTTTG |
TA, annealing temperature; IFN-γ, Interferon γ; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; IL-6, interleukin-6; ZO-1, zonula occludens-1; IKKβ, inhibiting kappa B kinase β; IκBα, inhibiting nuclear factor kappa-B; NF-κB, nuclear factor kappa-B.