Figure 4.

The zinc finger protein CLAMP is a strong interactor of MSL2^vir in vivo. (A) Volcano plot showing log₁₀ P-value in relation to the average log₂ fold change (n = 4 biological replicates) comparing the FLAG-MSL2^vir (MSL2^vir) AP-MS versus the control purification with FLAG-only beads (Ctrl). MSL2^vir is represented in red, other DCC subunits are represented in blue, and CLAMP is in orange. Heat shock proteins (green) are considered irrelevant bystander proteins. (B) Western blot analyses of coimmunoprecipitation (“pull-down”) experiments using the indicated MSL2-FLAG recombinant construct MSL2^mel (MEL) or MSL2^vir (VIR) as bait to retrieve proteins from extracts of either melanogaster or virilis cells. Five percent of the extracts was loaded as a reference (Input). “M” indicates the protein marker. (C) Western blot analyses of pull-down experiments as in B. Five percent of the extracts was loaded as a reference (Input). “M” indicates the protein marker. (D) Venn diagram showing overlapping peaks of MSL2^vir (VIR; GFP ChIP N = 2), MSL2^mel (MEL; GFP ChIP N = 2), and CLAMP (CLAMP ChIP from Kc cells N = 2) (from Soruco et al. 2013). Only peaks common to all replicates are considered.