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. 2021 Jul 1;35(13-14):1020–1034. doi: 10.1101/gad.348174.120

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© 2021 Soares et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Tetracysteine tag-based live-cell imaging confirms distinct abilities of Ascl1 and Brn2 to interact with mitotic chromosomes. (A–C) Representative captures from live-cell imaging of P19 cells expressing Ascl1/E47-eGFP (A), TC-Brn2-IRES-GFP (B), or TC-Ascl1/E47-IRES-GFP (C) in the presence of DNA dye SiR-Hoechst. (D) Quantifications of mitotic chromosome enrichment levels of all three conditions, measured using cells undergoing metaphase (n = 25 in Ascl1/E47-eGFP; n = 30 in TC-Brn2; n = 26 in TC-Ascl1/E47). Data shown as mean ± SD. (I) Interphase, (M) mitosis. Scale bars, 10µm.