Figure 2.

The neutralization activity of mAbs against 16 SARS-CoV-2 variants and mutations. (A) Monoclonal antibodies were serially diluted and mixed with equal amounts of the five different SARS-CoV-2 variants and those containing a single mutation. After pre-incubation at 37°C for 1 h, trypsinized Huh 7 cells were added. After cultivation for 24 h, the luminescence of the target cells was measured. The neutralization inhibition rate of the antibody and ID₅₀ was calculated using the Reed–Muench method. The data represent the ID₅₀ ratio of each variant to that of D614G. The neutralization ability of 19 different monoclonal antibodies (x axis) against 16 SARS-CoV-2 variants and mutations (y axis) are shown as heatmap. Red represents an increase in neutralization capacity, while blue represents a decrease in neutralization capacity. Four-fold changes were considered statistically significant. (B) Structure modeling of the mutation N501Y in S1 and S982A in S2 based on “6VXX”.