Figure 2.

Phosphorolytic activity of TtPyNP in the presence of the organic cosolvents dimethyl sulfoxide (DMSO, A) and ethylene glycol (B). Reactions were run with 1 mM 1 a, 20 mM potassium phosphate and 0.12 or 0.40 μg mL⁻¹ TtPyNP in 20 mM glycine/NaOH buffer pH 9 containing the indicated amount of cosolvent at the indicated temperature. Data shown represent the average of two experiments. Error bars are too small to see and were omitted for clarity. Please see the Supporting Information for more experimental details and the externally hosted Supporting Information for raw data and calculations. ^[20]